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Study note

Effects of caffeine and its metabolites, theobromine and theophylline, on performance and physiological parameters in cyclists: A randomized, double-blind, placebo-controlled, crossover trial.

PMID 40850526 (2025): caffeine — Time-trial performance, Time to exhaustion (study note for endurance athletes).

Updated Jul 22, 2026

Read the study note

Study note • PMID 40850526

Effects of caffeine and its metabolites, theobromine and theophylline, on performance and physiological parameters in cyclists: A randomized, double-blind, placebo-controlled, crossover trial.

Clinical nutrition ESPEN2025 • DOI 10.1016/j.clnesp.2025.08.021

How to use this page

This note summarizes one study record. Check the participants, methods, results, and limitations before applying it to training.

  • Design signal: randomized trial.
  • Population relevance: mixed/unclear athlete specificity.
  • Consistency: single-study evidence.
  • Practical recommendation depends on tolerance, context, and whether it protects training consistency.

ELI5

In plain language

This study aimed to evaluate the effects of caffeine and its metabolites, particularly theobromine and theophylline, on physiological and performance parameters in recreational cyclists. (randomized trial; recreational cyclists).

The abstract doesn’t indicate a clear change in Time-trial performance under the tested conditions. Treat this as a signal, not a guarantee; confirm methods and context in the full paper.

Takeaways

What the abstract suggests

  • Study question: This study aimed to evaluate the effects of caffeine and its metabolites, particularly theobromine and theophylline, on physiological and performance parameters in recreational cyclists.
  • The abstract doesn’t indicate a clear change in Time-trial performance under the tested conditions.
  • Population: recreational cyclists.
  • Protocol cues (title/abstract): 6 mg • 16 km.

Protocol

Protocol (as reported)

  • Intervention/exposure: caffeine (vs placebo).
  • Dose/time/duration cues in abstract/title: 6 mg • 16 km.
  • Outcomes: Time-trial performance, Time to exhaustion.
  • Replication note: abstracts often omit adherence and timing; confirm details before changing training or supplementation.

Fit

Who it may help, and who should be cautious

Who it helps

  • Athletes similar to the study population (recreational cyclists) working on supplements.
  • Athletes who can measure Time-trial performance, Time to exhaustion with a repeatable workout or time-trial effort.

Who should be cautious

  • If you have symptoms or conditions that make the intervention risky, get professional guidance.
  • If you’re near race day and can’t safely test, defer the experiment.

Methods

What the study actually did

  • Design: randomized trial (double-blind, placebo-controlled).
  • Population: recreational cyclists.
  • Comparator: placebo.
  • Outcomes measured: Time-trial performance, Time to exhaustion.
  • Protocol cues mentioned: 6 mg • 16 km.
  • Source: PubMed PMID 40850526 (2025) — Clinical nutrition ESPEN.

Results excerpt

What the abstract reports

The theophylline/caffeine ratio showed a significant correlation with VE (r = -0.69, p < 0.05) and TT duration (r = 0.73, p < 0.01).

Note: excerpts are short; for full context, read the paper.

Limits

Limitations and bias

  • Abstract-only summaries can miss critical details (population, protocol, adherence, and context).
  • Single studies often don’t generalize to your event, history, and training load; treat results as a starting point.
  • If your context differs (elite vs recreational; cycling vs running), adjust expectations and be conservative.
  • This is performance information, not medical advice.

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