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Acute caffeine intake improves muscular strength, power, and endurance performance, reversing the time-of-day effect regardless of muscle activation level in resistance-trained males: a randomized controlled trial.

PMID 40478455 (2025): caffeine — Time-trial performance, Time to exhaustion (study note for endurance athletes).

Last updated/Feb 23, 2026, 10:34 PM

Study note • PMID 40478455

Acute caffeine intake improves muscular strength, power, and endurance performance, reversing the time-of-day effect regardless of muscle activation level in resistance-trained males: a randomized controlled trial.

European journal of applied physiology2025 • DOI 10.1007/s00421-025-05820-3
Evidence B70/100
Action 2: Consider

Worth trying if it fits your goal and context.

ELI5

In plain language

INTRODUCTION: This study examined the effects of acute caffeine intake on muscular electrical activity during strength, power, and endurance performance tests at different times of day in bench press… (randomized trial; trained participants).

The abstract doesn’t indicate a clear change in Time-trial performance under the tested conditions. Treat this as a signal, not a guarantee; confirm methods and context in the full paper.

Takeaways

What the abstract suggests

  • Study question: INTRODUCTION: This study examined the effects of acute caffeine intake on muscular electrical activity during strength, power, and endurance performance tests at different times of day in bench press…
  • The abstract doesn’t indicate a clear change in Time-trial performance under the tested conditions.
  • Population: trained participants.
  • Protocol cues (title/abstract): 3 mg/kg • 60 min.

Protocol

Protocol (as reported)

  • Intervention/exposure: caffeine (vs placebo).
  • Dose/time/duration cues in abstract/title: 3 mg/kg • 60 min.
  • Outcomes: Time-trial performance, Time to exhaustion.
  • Replication note: abstracts often omit adherence and timing; confirm details before changing training or supplementation.

Fit

Who it helps, and who should skip it

Who it helps

  • Athletes similar to the study population (trained participants) working on supplements.
  • Athletes who can measure Time-trial performance, Time to exhaustion with a repeatable workout or time-trial effort.

Who should skip

  • If you have symptoms or conditions that make the intervention risky, get professional guidance.
  • If you’re near race day and can’t safely test, defer the experiment.

Methods

What the study actually did

  • Design: randomized trial (placebo-controlled).
  • Population: trained participants.
  • Comparator: placebo.
  • Outcomes measured: Time-trial performance, Time to exhaustion.
  • Protocol cues mentioned: 3 mg/kg • 60 min.
  • Source: PubMed PMID 40478455 (2025) — European journal of applied physiology.

Results excerpt

What the abstract reports

At 25%1RM, caffeine counteracts morning performance decline in bench press (10-11%, P = 0.001, g = 2.62-1.68) and back squat (8-11%, P = 0.010-0.003, g = 2.22-1.64).

Note: excerpts are short; for full context, read the paper.

Limits

Limitations & bias

  • Abstract-only summaries can miss critical details (population, protocol, adherence, and context).
  • Single studies often don’t generalize to your event, history, and training load; treat results as a starting point.
  • If your context differs (elite vs recreational; cycling vs running), adjust expectations and be conservative.
  • This is performance information, not medical advice.

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Sources