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Effects of Protein Supplementation During High-Intensity Functional Training on Physical Performance in Recreationally Trained Males and Females: A Randomized Controlled Trial.

PMID 40362749 (2025): aerobic, endurance — VO₂max, Lactate threshold (study note for endurance athletes).

Last updated/Feb 23, 2026, 10:34 PM

Study note • PMID 40362749

Effects of Protein Supplementation During High-Intensity Functional Training on Physical Performance in Recreationally Trained Males and Females: A Randomized Controlled Trial.

Nutrients2025 • DOI 10.3390/nu17091441
Evidence B71/100
Action 1: Default

Low risk + high feasibility for most athletes.

ELI5

In plain language

High-intensity functional training (HIFT) combines multijoint aerobic and resistance exercises. (randomized trial; trained participants).

The abstract doesn’t indicate a clear change in VO₂max, Lactate threshold under the tested conditions. Treat this as a signal, not a guarantee; confirm methods and context in the full paper.

Takeaways

What the abstract suggests

  • Study question: High-intensity functional training (HIFT) combines multijoint aerobic and resistance exercises.
  • The abstract doesn’t indicate a clear change in VO₂max, Lactate threshold under the tested conditions.
  • Population: trained participants.
  • Protocol cues (title/abstract): 0.6 g/kg • 1.0 g/kg • 1.6 g/kg • 6 weeks • 2 weeks.

Protocol

Protocol (as reported)

  • Intervention/exposure: aerobic, endurance (vs placebo).
  • Dose/time/duration cues in abstract/title: 0.6 g/kg • 1.0 g/kg • 1.6 g/kg • 6 weeks • 2 weeks.
  • Outcomes: VO₂max, Lactate threshold.
  • Replication note: abstracts often omit adherence and timing; confirm details before changing training or supplementation.

Fit

Who it helps, and who should skip it

Who it helps

  • Athletes similar to the study population (trained participants) working on endurance.
  • Athletes who can measure VO₂max, Lactate threshold with a repeatable workout or time-trial effort.

Who should skip

  • If you have symptoms or conditions that make the intervention risky, get professional guidance.
  • If you’re near race day and can’t safely test, defer the experiment.

Methods

What the study actually did

  • Design: randomized trial (placebo-controlled).
  • Population: trained participants.
  • Comparator: placebo.
  • Outcomes measured: VO₂max, Lactate threshold.
  • Protocol cues mentioned: 0.6 g/kg • 1.0 g/kg • 1.6 g/kg • 6 weeks • 2 weeks.
  • Source: PubMed PMID 40362749 (2025) — Nutrients.

Results excerpt

What the abstract reports

Short-term HIFT improved exercise capacity, upper-body strength, and core endurance.

Note: excerpts are short; for full context, read the paper.

Limits

Limitations & bias

  • Abstract-only summaries can miss critical details (population, protocol, adherence, and context).
  • Single studies often don’t generalize to your event, history, and training load; treat results as a starting point.
  • If your context differs (elite vs recreational; cycling vs running), adjust expectations and be conservative.
  • This is performance information, not medical advice.

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Sources